EXPERIENCE WITH
Please, mark yes ONLY if you are really trained and able to work independently in a given area. Being just exposed to a given field is not sufficient.
DNA cloning
Please, specify in the window on the right, if you have experience with any of the following: preparation of recombinant plasmids, production of plasmid libraries (cDNA, miRNA, genomic), cosmids, BACs, YACs.
Cell culture
Please, specify in the window on the right, if you have experience with any of the following: preparing primary cell lines, culturing transformed cell lines, transfection, electroporation, preparation of stably transformed (transgenic) cell lines, embryonic stem cells, use of homologous recombination in embryonic stem cells.
Recombinant proteins
Please, specify in the window on the right which type of expression system and purification you used.
Antibody production
Please, specify in the window on the right, if you have experience with antigen preparation (peptide design, recombinant protein production), immunization (which organism), antibody purification and testing.
Microscopy
Please, specify in the window on the right your experience with sample preparation (e.g. embedding, cryofixation and cryosections), staining method, and microscopic method for sample observation (SEM, TEM, STEM, light sheet, time-lapse etc.).
Simulations & Modelling
Please, specify in the window on the right, if you have experience with mathematical simulations, DNA/RNA/protein structural predictions or any other type of computer, mathematical or physical modelling.
Programming
Please, specify in the window on the right which system (Unix, Linux, Windows…) and language (Perl…).
RNA- & DNA-seq analysis
Please, specify in the window on the right which sequencing method and platform did you use, which part of the analysis have you been involved in (sample collection, library preparation, sequencing, raw data processing, data interpretation and analysis), and what tools you utilized for the analysis.
Radioactivity
Please, specify in the window on the right which isotope(s) did you work with. Do you have any official recognition of being trained to work with radioactivity?
Animal work
Please, specify in the window on the right which type of animal work you
performed: animal model, species, breeding, numbering and tissue biopsies for
genotyping, preimplantation embryo collection, post implantation embryo
collection and analysis, tissue preparation (which tissues). Do you have
any official recognition of being trained to work with animals?
Non-vertebrate models
Please, specify in the window on the right which model (bacterial, plants,
C. elegans, Drosophila etc.).